The ACQUITY UPLC M-Class System is engineered for high-performance nano- to micro-scale separations, delivering exceptional sensitivity for sample-limited proteomics and biomarker discovery workflows. Operating from 200 nL/min to 100 µL/min without flow splitting, it provides pulse-free delivery for excellent retention time stability. With a 15,000 psi pressure limit and compatibility with columns from 75 µm to 1.0 mm ID, it supports a wide range of applications. Its non-reactive flow path and 4°C to 40°C temperature control preserve sample integrity. The system integrates seamlessly with UV and MS detection, and supports HDX, 2D separations, and advanced analytical configurations.