Baxter guides surgeons and OR staff through the correct preparation and spray‑application of TISSEEL fibrin sealant using the DUPLOSPRAY MIS regulator — ideal for minimally invasive surgery (MIS). The video walks through thawing and warming the frozen TISSEEL dual‑chamber syringe, connecting the spray set to the DUPLOSPRAY regulator, attaching the applicator to the syringe, and preparing medical‑grade CO₂ as the driving gas. It further demonstrates the controlled application: activating the gas via foot‑pedal, depressing the syringe plunger slowly and steadily, spraying at the recommended distance (approx. 2–5cm from tissue), and clearing the applicator tip post‑spray to avoid clogging. The protocol emphasises safety — including proper pressure settings, gas flow checks, and adherence to tissue‑surface‑only application — ensuring effective hemostasis or sealing while minimizing risks such as gas embolism or sealant denaturation.